Beer–Lambert Law Calculator

Find absorbance with the Beer–Lambert law.

How to use

  1. Enter your values in the fields above.
  2. Press Calculate to see your result instantly.
  3. Use the Share button to copy a link to your result.

About this calculator

This calculator applies the Beer–Lambert law to find absorbance: A = ε·b·c, where ε is the molar absorptivity (extinction coefficient, L·mol⁻¹·cm⁻¹), b is the path length through the sample (cm), and c is the molar concentration. It also converts the result to percent transmittance: %T = 10^(2−A).

Analytical chemists, biochemists, and lab technicians use this relationship constantly with UV-Vis spectrophotometers to determine an unknown sample's concentration from its measured absorbance, or to predict the absorbance expected from a known concentration and cuvette path length. Enter any two of ε, b, and c to solve for absorbance and transmittance.

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